抑制血红素氧合酶-1对大鼠免疫性肝纤维化的影响

世界华人消化杂志, 2009; 17(6) : 560-565.

 

【中文关键词】	血红素氧合酶; 锌原卟啉; 肝纤维化; 大鼠;
【英文关键词】	Hemeoxygenases-1; Zinc protoporphyrin; Hepatic fibrosis; Rat;
【摘要】	目的:逆向观察肝纤维化大鼠血红素氧合酶-1(heme oxygenase1,HO-1)的表达与肝纤维化形成的关系,探讨HO-1对免疫性肝纤维化大鼠肝脏的保护作用.方法:应用人体血清白蛋白建立免疫性肝纤维化模型,Zn组在攻击阶段同时腹腔注射Znpp,HE染色、VG染色、Foot氏网状纤维染色等三种染色方法观察肝纤维组织增生程度,Western blot和免疫组化法观察肝组织中HO-1的表达.结果:F组和Zn组免疫组化阳性计分较N组明显升高(4.00±1.31,2.33±0.78vs0.80±0.79,P<0.01);且F组免疫组化阳性计分比Zn组高(4.00±1.31vs2.33±0.78,P<0.05).F组成纤维细胞、Ⅰ型胶原及Ⅲ型胶原增生较N组明显(P<0.01);Zn组成纤维细胞、Ⅰ型胶原及Ⅲ型胶原增生较F组更明显(P<0.01).结论:免疫性肝纤维化模型中,肝脏能够高水平表达HO-1,对肝脏起保护作用.
【英文摘要】	AIM:To observe effects of hemeoxygenase-1(HO-1) on immune hepatic-fibrosis in rats.METHODS:The hepatic fibrosis model was induced by human serum albumin(HSA).Znpp group was administrated ZnPP intraperitoneally at attacking stage.The fibrous hyperplasia degree were observed by HE staining,VG staining and Foot"s reticular fiber staining.The expressions of HO-1 protein in liver were measured by immunohistochemistry and Western blot.RESULTS:The positive scores ofimmunohistochemistry in F group and Zn group were...
【基金】大连医科大学附属第一医院消化内科段志军	国家自然科学基金资助项目,No.2005-30570515; 教育部留学回国人员科研启动基金资助项目,No.2005-546; 辽宁省教育厅基金资助项目,No.2004-F063~~ http://epub.cnki.net/grid2008/detail.aspx?DBName=CJFD0911&FileName=XXHB200906008