Wilms' tumor gene (WT1) is predominantly expressed in clonal hematopoietic cells in myelodysplastic syndromes.

Leuk Lymphoma. 2007 Mar;48(3):601-4.

 

Li X,Wu L, Ying S, Chang C,Pu Q.

Department of Hematology, The Sixth People"s Hospital Affiliated to Shanghai Jiaotong University, Shanghai, China. [e-mail]

To determine the expression of Wilms" tumor gene (WT1) in clonal hematopoietic cells in patients with myelodysplastic syndromes (MDS), immunochemistry labelling (alkaline phosphatase anti-alkaline phosphatase) and fluorescence in situ hybridization (FISH) were coperformed on bone marrow cytospins from 18 patients whose abnormal karyotypes had been determined by G-binding analysis. Compared to 12 healthy donors, WT1 positive nucleated cells in MDS marrows were significantly higher (t = 2.30; P = 0.032). Moreover, WT1 was expressed predominantly in MDS clonal cells (with abnormal FISH signals) rather than in non-clonal cells (residual normal cells) (t = 2.19; P = 0.043).上海市第六人民医院血液内科常春康