-
- 张磊涛副主任医师 博士
-
医院:
南方医科大学南方医院
科室:
口腔科
- Tissue inhibitor of metalloproteinase-2 inhibits ameloblastoma growth in a new mouse xenograft disease model
- 作者:张磊涛|发布时间:2013-06-21|浏览量:676次
background: ameloblastomas are odontogenic
neoplasms characterized by local invasiveness. this study
was conducted to develop a new animal model of
ameloblastoma and to address the role of tissue inhibitor
of metalloproteinase-2 (timp-2) and matrix metalloproteinase-南方医科大学南方医院口腔科张磊涛
2 (mmp-2) in the growth and invasiveness of
ameloblastomas.
method: donated fresh human ameloblastoma tissue
was finely minced, screened, and subcutaneously
implanted in three locations on each of 10 balb? c-nu ? nu
nude mice. newly established tumors on each mouse
were injected with: (i) transfection reagent; (ii) liposome
and transfection reagent; or (iii) liposome, transfection
reagent, and the expression plasmid pcdna3.1(+) ? green
fluorescent protein (gfp)-timp-2. tumors were monitored
for 5 weeks and excised for histopathology, rna,
and protein analyses.
results: the ameloblastoma xenografts were established
with high frequency and contained a variety of
typical features, validating this new model system.
xenografts injected with the timp-2 expression plasmid
showed reduced growth, increased timp-2 mrna and
protein, and decreased mmp-2 protein compared with
the control groups.
conclusions: we successfully established a new
experimental model of ameloblastoma consisting of
subcutaneous human xenografts in nude mice. in addition,
we demonstrated the successful introduction of the
timp-2 gene in tumor xenograft cells in vivo, resulting in
xenograft growth inhibition. this growth inhibition may
have resulted from timp-2 overexpression specifically
inhibiting mmp-2 protein expression and activity.
j oral pathol med (2010) 39: 94?102
neoplasms characterized by local invasiveness. this study
was conducted to develop a new animal model of
ameloblastoma and to address the role of tissue inhibitor
of metalloproteinase-2 (timp-2) and matrix metalloproteinase-南方医科大学南方医院口腔科张磊涛
2 (mmp-2) in the growth and invasiveness of
ameloblastomas.
method: donated fresh human ameloblastoma tissue
was finely minced, screened, and subcutaneously
implanted in three locations on each of 10 balb? c-nu ? nu
nude mice. newly established tumors on each mouse
were injected with: (i) transfection reagent; (ii) liposome
and transfection reagent; or (iii) liposome, transfection
reagent, and the expression plasmid pcdna3.1(+) ? green
fluorescent protein (gfp)-timp-2. tumors were monitored
for 5 weeks and excised for histopathology, rna,
and protein analyses.
results: the ameloblastoma xenografts were established
with high frequency and contained a variety of
typical features, validating this new model system.
xenografts injected with the timp-2 expression plasmid
showed reduced growth, increased timp-2 mrna and
protein, and decreased mmp-2 protein compared with
the control groups.
conclusions: we successfully established a new
experimental model of ameloblastoma consisting of
subcutaneous human xenografts in nude mice. in addition,
we demonstrated the successful introduction of the
timp-2 gene in tumor xenograft cells in vivo, resulting in
xenograft growth inhibition. this growth inhibition may
have resulted from timp-2 overexpression specifically
inhibiting mmp-2 protein expression and activity.
j oral pathol med (2010) 39: 94?102