- Human umbilical cord blood cells express neurotrophic factors
- 作者:范存刚|发布时间:2009-10-07|浏览量:1004次
Human umbilical cord blood cells express neurotrophic factors北京大学人民医院神经外科范存刚
Cun-Gang Fan1, 2, Qing-Jun Zhang2, Feng-Wu Tang1,
Zhi-Bo Han1, Ge-Sheng Wang2, and Zhong-Chao Han1
1. National Research Center for Stem Cell Engineering & Technology, State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, People’s Republic of China
2. Department of Neurosurgery, the Second Hospital of Hebei Medical University, Shijiazhuang, People’s Republic of China
Correspondence and reprint requests: Dr. Zhong Chao Han, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300020, PR China;
Tel: 86 22 27317276; Fax: 86 22 27317273; e-mail: tihzch@public.tpt.tj.cn
The manuscript includes 17 pages, 1 figures and 1 tables.
Acknowledgements: This work was supported by grants of 863 (2002AA217041, 2002AA223354, 2003AA205060) and 973 (001CB5101) projects from the Ministry of Science & Technology of China, and a grant from the China Medical Board of New York (#01-748) to Z. C. Han.
Abstract
Freshly isolated or culture-expanded human umbilical cord blood mononuclear cells (CBMNCs) have been known to express neural phenotypes in vitro and to differentiate into neural cells and improve neurological function recovery after being administrated into rodent models of neurological diseases. However, the mechanism of action remains unclear. The present study observed that CBMNCs expressed higher level mRNAs of several neurotrophic factors than adult peripheral blood mononuclear cells (PBMCs). In addition, a significantly increase in the levels of brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT4/5) was found in culture supernatants of CBMNCs compared to that of PBMNCs. These findings indicate that CBMNCs express several neurotrophic factors and suggest that the neurotrophic factors secreted by CBMNCs may be responsible for amelioration of central nervous system deficits in animal models after CBMNC administration.