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- 异基因和同基因骨髓移植/造血干细胞移植供者准备(英语版)
- 作者:万鼎铭|发布时间:2009-06-10|浏览量:2209次
Evaluation 1. Current and past medical problems. H&P must be completed within seven days of surgery (consulting hour) or updated; updates may be done by telephone. If the H&P is thirty days or older, it will need to be re-done.(H&P = history and physical examination).郑州大学一附院血液内科万鼎铭
2. Current medications.
3. History of previous transfusions.
4. Female donors: Pregnancy history (teenage and adults only).
5. Completed donor health status questionnaire.
Clinical Evaluation 1. Physician examination.
2. Height and weight.
3. Phlebotomy and Venipuncture/Venepuncture at Anesthesiology Department and ICU (intensive care unit) as appropriate sites.
4. ECG if donor has history or falls within any of the following categories: History of: ①Diabetes ②Heart disease ③Vascular surgery ④Emphysema or chronic obstructive lung disease ⑤Hypertension ⑥Is smoker (donor) older than 40? ⑦Is donor older than 50?
Laboratory Evaluation 1. CBC.
2. Biochemical Tests: Na (natrium/sodium), K (kalium/potassium), Cl (chlorine), Ca (calcium), Phos (phosphorus), Mg (magnesium); Glu (Glucosis), BUN (blood urea nitrogen), CO2CP (carbon dioxide combining power), UA (uric acid), Cr (creatinine); ALT (SGPT), AST (SGOT), A (albumin), G (globulin), A/G ratio, TP (total protien), TB (total bilirubin), DB (direct/conjugated bilirubin), IB (indirect/unconjugated bilirubin), LDH (lactate dehydrogenase), GGT (gamma glutamyl transpeptidase), ALP (alkaline phosphatase); Tg (triglycerides), Chol (cholesterol), et al.
3. ABO, Rh typing and two-way red cell crossmatch with patient.
4. Obtain the following as requested by HLA: ①HLA serotyping ②DNA sequence specific oligonucleotide probe (SSOP) typing ③For patient and donor who are suspected of being monozygotic twins, 20 ml heparinized peripheral blood for DNA-VNTR(variable number tandem repeats) analysis ④Leukocytotoxic and/or FACS(Fluorescence-activated cell sorter) crossmatch between the patient and donor ⑤For patient with sex-matched donor in whom chimerism cannot be documented by in situ hybridization, 20 ml heparinized peripheral blood from patient and donor for DNA storage.
5. 10 ml clot tube for storage (3 ml for pediatric).
6. 3 ml for cytogenetic studies.
7. Hepatitis testing done: HbsAg, Anti-HBc, ALT, Anti-HIV, Anti-HTLV-1, STS, Anti-HCV (all tests part of the hepatitis battery). Tests must be completed within thirty days of surgery or repeated.
8. Female donors of menstruation age (>10 years): Serum HCG (human chorionic gonadotropin).
Medications 1. Ferrous succinate 1~2 tablets (100 mg each) PO TID (Adult).
2. Ferrous succinate 1 tablets (100 mg each) PO QD~BID (pediatric).
3. Vitamin C (Ascorbic acid) 1~2 tablets (100 mg each) PO TID (Adult).
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